Wolverine Stack Dosage & Reconstitution Guide (BPC-157 + TB-500) Blended & Separate Vial Protocols

Wolverine Stack (BPC-157 + TB-500)

The Wolverine Stack combines BPC-157 and TB-500 in one recovery-focused protocol designed to support tissue repair, healing, inflammation control, and systemic recovery. BPC-157 is often discussed for localized repair signaling, while TB-500 is known for broader healing and cell-migration support.

How It Works

BPC-157 → Local Tissue Repair + Blood Flow Support
TB-500 → Cell Migration + Systemic Healing Support
Combined → Faster Recovery + Better Tissue Repair

This stack is commonly discussed for tendon, ligament, muscle, joint, and soft-tissue recovery. The blended version is simpler and more beginner-friendly, while the separate vial version allows BPC-157 and TB-500 to follow different frequency patterns.

Blended Vial Protocol (Simple)

5 mg BPC-157 + 5 mg TB-500 → 3.0 mL BAC Water → 1 unit = 16.7 mcg of each peptide

Blended Vial Setup

Blend5 mg BPC-157 + 5 mg TB-500

Water3.0 mL

Concentration1.67 mg/mL each

Conversion1 unit = 16.7 mcg BPC-157 + 16.7 mcg TB-500

Combined1 unit = 33.3 mcg total

Weeks 1–2

Loading

Dose250 mcg of each peptide daily

Combined Total500 mcg total

Units15 units total

Volume0.15 mL

Weeks 3–4

Peak Repair

Dose500 mcg of each peptide daily

Combined Total1,000 mcg total

Units30 units total

Volume0.30 mL

Weeks 5–8

Maintenance

Dose500 mcg of each peptide daily

Combined Total1,000 mcg total

Units30 units total

Volume0.30 mL

Why use blended?

Simple Structure

This is the simplest version. Both peptides are delivered together in one syringe with one daily draw.

Best For

Great for convenience, easier math, and a more beginner-friendly protocol flow.

Separate Vials Protocol (Advanced)

BPC-157 = Daily Repair Signaling → TB-500 = Less Frequent Systemic Support → More Flexible Dosing Logic

Separate Vials Setup

Based on 10 mg vials for each peptide

BPC-15710 mg vial + 3.0 mL BAC water

TB-50010 mg vial + 3.0 mL BAC water

BPC Conversion1 unit = 33.3 mcg

TB Conversion1 unit = 33.3 mcg

Weeks 1–2

Loading

BPC-157250 mcg daily = 7.5 units daily

Volume0.075 mL daily

TB-5002 mg, 2x weekly = 60 units each dose

Volume0.60 mL each TB dose

If Drawn Together on TB Day67.5 units total = 0.675 mL

Weeks 3–4

Peak Repair

BPC-157500 mcg daily = 15 units daily

Volume0.15 mL daily

TB-5002 mg, 2x weekly = 60 units each dose

Volume0.60 mL each TB dose

If Drawn Together on TB Day75 units total = 0.75 mL

Weeks 5–8

Maintenance

BPC-157500 mcg daily = 15 units daily

Volume0.15 mL daily

TB-5002 mg weekly = 60 units once weekly

Volume0.60 mL on TB day

If Drawn Together on TB Day75 units total = 0.75 mL

Why use separate vials?

More Control

This setup keeps BPC-157 on a daily schedule while allowing TB-500 to follow a lower-frequency systemic pattern.

Best For

More flexible and usually feels more aligned with how TB-500 is commonly structured in research discussions.

How to Use the Wolverine Stack (Cycle, Timing & Injection)

Cycle Length4–8 weeks

Break Between Cycles2–4 weeks off

Injection TypeSubcutaneous

Use StyleBlended or separate vials

Best Time to Take

Any consistent time of day can work

Blended version is typically once daily

Separate-vial version often follows daily BPC-157 with less frequent TB-500

Consistency matters more than exact timing

Where to Inject

Abdomen, thigh, or upper arm

BPC-157 is often used near the injury site in localized protocols

TB-500 is often discussed as more systemic, so injection location is less specific

You can draw both peptides into one syringe or inject separately depending on preference

When to Use

During injury, tendon, ligament, or muscle recovery phases

When inflammation and soreness are elevated

During soft-tissue repair support periods

As a shorter-term recovery-focused protocol rather than continuous use

Simple Strategy

Blended

Best for simplicity and one-syringe convenience.

Separate

Best for more control over BPC-157 and TB-500 frequency and timing.

Blended vs Separate (Which Should You Use?)

Blended Vial

Best for simplicity and one-syringe convenience

Both peptides follow the same daily schedule

Easy math and beginner-friendly structure

Separate Vials

Best for advanced use and more flexible timing

BPC-157 can remain daily while TB-500 is used less often

Better reflects how TB-500 is often structured in research protocols

Simple takeaway:
Choose blended if you want simplicity. Choose separate if you want more control over BPC-157 vs TB-500 frequency.

Storage & Shelf Life

Storage & Shelf Life

Lyophilized (unmixed)

Refrigerated (2–8°C): ~12–24 months

Frozen (−20°C): up to ~2–3 years

Reconstituted (mixed)

Refrigerated (2–8°C): ~30 days typical use window

Do not freeze after mixing

Blended vs Separate Storage

Blended Vials

Both peptides share the same solution, so stability is tied together. If one begins degrading, the entire vial is affected.

Separate Vials

Each peptide maintains its own stability. This is slightly more flexible and can preserve integrity better over time.

Best Practice

Handling

Keep vials cold and protected from light.

Temperature

Avoid repeated temperature changes.

Visual Check

If solution becomes cloudy, discolored, or has particles, discard.

Technique

Use clean injection technique to help prevent contamination.

Monitoring During Use

Injury healing progress

Joint, tendon, or muscle recovery

Inflammation and soreness

Injection site tolerance

Benefits

Supports tissue repair and healing

May improve tendon and ligament recovery

Supports angiogenesis and cell migration

Often discussed for joint, soft tissue, and injury recovery

Potential Side Effects

Mild injection site irritation

Redness or swelling

Limited human clinical data

Important Notes

Blended vial math is based on 5 mg + 5 mg reconstituted with 3.0 mL total

Separate vial math is based on 10 mg BPC-157 and 10 mg TB-500 reconstituted with 3.0 mL each

250 mcg each = 500 mcg combined total

500 mcg each = 1,000 mcg combined total

Separate vials allow BPC-157 and TB-500 to follow different dosing frequencies

Important Disclaimer

BPC-157 and TB-500 are investigational compounds and are not FDA-approved. This content is for educational and research purposes only.

Wolverine Stack Guide → Peptide Directory →