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GHK-Cu Dosage & Reconstitution Guide: mg to mcg Chart

GHK-Cu Peptide

GHK-Cu (glycyl-L-histidyl-L-lysine copper complex) is a naturally occurring copper-binding tripeptide studied for its role in wound healing, tissue remodeling, collagen synthesis, and inflammatory signaling. Preclinical and topical research suggests GHK-Cu may influence pathways involved in regeneration, antioxidant defense, and skin repair.

How GHK-Cu Works

Collagen ↑ → Tissue Repair ↑ → Inflammation ↓ → Skin & Hair Regeneration ↑

GHK-Cu is often discussed for skin quality, wound healing, connective tissue repair, and hair support. It is commonly used in both cosmetic-focused and healing-focused protocols, with dose ranges varying by goal.

Typical Injectable Research Protocol (5 Days / Week)
50 mg vial → 3.0 mL BAC Water → 1 unit = 167 mcg
Standard Setup
Vial 50 mg
Water 3.0 mL bacteriostatic water
Concentration 16.67 mg/mL
Conversion 1 unit = 166.7 mcg
Weeks 1–4
Assess Tolerance
Dose1.0 mg (1000 mcg)
Units6 units
Volume0.06 mL
Weeks 5–8
Increased Response
Dose1.5 mg (1500 mcg)
Units9 units
Volume0.09 mL
Weeks 9–12
Full Protocol
Dose2.0 mg (2000 mcg)
Units12 units
Volume0.12 mL
GHK-Cu is often used in a gradual build structure. Lower ranges are commonly used for skin-focused support, while higher ranges are more often discussed for connective tissue or injury-focused protocols.
Alternative Protocol (3× Weekly)
Weeks 1–12+
Simplified Protocol
Dose2.0 mg (2000 mcg)
Units12 units
Volume0.12 mL
Frequency3× weekly
This lower-frequency structure is sometimes discussed as an alternative research-use approach. Direct human evidence comparing injectable GHK-Cu dosing frequencies has not been established.
How to Use GHK-Cu (Cycle, Timing & Injection)
Standard FrequencyOnce daily, 5 days per week
Alternative Frequency3× weekly
Injection TypeSubcutaneous
Cycle LengthOften 8–12+ weeks
Time Between Cycles
Standard Cycle8–12 weeks
Time Off2–4 weeks
Extended Option12–16 weeks, then 4 weeks off
Simple takeaway:
GHK-Cu is usually run in 8–12 week support phases, followed by 2–4 weeks off. Longer cosmetic or tissue-repair phases may extend to 12–16 weeks before taking a break.
Best Time to Take
Consistent timing matters more than exact time of day
Morning or evening can both work
Many use it at a consistent daily time for easier tracking
Topical or cosmetic-focused users may align timing with their broader skin-care routine
Where to Inject
Abdomen (most common)
Thigh or upper arm
Localized injection near a target area is sometimes discussed in research-use protocols, although human evidence has not established an advantage to injecting GHK-Cu near the target tissue.
Rotate injection sites regularly
When to Use
For skin-focused support phases
During tissue repair or recovery periods
When hair or follicle support is the main goal
As a longer regenerative-support phase rather than a short aggressive cycle
Simple Strategy
Goal-Based Use
Lower doses are often used for skin and cosmetic support, while higher doses are more often discussed for injury or deeper tissue repair.

Consistency
GHK-Cu is usually more about steady regenerative support over time than fast noticeable changes.
Reconstitution & Dosing (50 mg Vial)
Reconstitution & Dosing
Vial50 mg
Water3.0 mL bacteriostatic
Concentration16.67 mg/mL
Conversion1 unit = 167 mcg
Common Conversions
1 mg = 6 units (0.06 mL)
1.5 mg = 9 units (0.09 mL)
2 mg = 12 units (0.12 mL)
Reconstitution Steps
Draw 3.0 mL bacteriostatic water
Inject slowly down vial wall
Gently swirl (do not shake)
Refrigerate immediately
Protect from light
Storage & Shelf Life
Storage & Shelf Life
Lyophilized (unmixed)
Refrigerated (2–8°C): ~12–24 months
Frozen (−20°C): up to ~2–3 years
Reconstituted (mixed)
Refrigerated (2–8°C): ~30 days typical use window
Avoid freezing after mixing (can degrade structure)
Important
Lyophilized Stability
Lyophilized GHK-Cu is relatively stable when kept cold and protected from light. Freezing significantly slows degradation and is best for long-term storage.
After Reconstitution
Once reconstituted, stability drops more quickly. Refrigeration and minimal handling help preserve potency.
Potential / Studied Benefits
Studied for collagen production and skin-remodeling pathways
May support wound-healing and tissue-repair processes
Studied for potential effects on hair and follicle health
Demonstrates antioxidant and anti-inflammatory activity in preclinical research
Studied for its role in tissue remodeling and regenerative signaling
Potential Side Effects / Safety Considerations
Skin irritation or sensitivity may occur with topical use
Injection-site redness or irritation may occur with injectable use
Human safety data for systemic/injectable GHK-Cu remain limited
Long-term safety of repeated injectable use has not been established
Stacking Strategies
GHK-Cu → Collagen & Tissue Repair
BPC-157 → Healing & Blood Flow
TB-500 → Systemic Recovery
Skin: GHK-Cu + microneedling / topical routine
Healing: GHK-Cu + BPC-157
Advanced: GHK-Cu + BPC-157 + TB-500
Important Notes
Research on GHK-Cu includes preclinical, topical, and cosmetic applications, but these evidence bases should not be treated as equivalent to injectable use.
Human evidence supporting specific subcutaneous GHK-Cu doses, frequencies, or cycle lengths remains limited.
Injectable protocols discussed on this page reflect research-use and community-practice frameworks rather than validated clinical dosing guidelines.
Longer protocols should be reassessed rather than assumed to require continuous use.

Important Disclaimer

GHK-Cu is investigational and not FDA-approved. Educational use only.

Research & References

Primary Research

Research Note: GHK-Cu has one of the longer research histories among cosmetic and repair-focused peptides, with decades of laboratory, animal, and topical human studies supporting its role in tissue remodeling, collagen synthesis, and antioxidant/anti-inflammatory activity. However, most of this evidence involves topical formulations (creams, serums) rather than subcutaneous injection, so reconstitution and dosing protocols for injectable use are drawn from general peptide-handling practice rather than dedicated injectable-GHK-Cu clinical trials.
GHK-Cu Guide → Peptide Directory →
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